Oxidative Medicine and Cellular Longevity Volume 2018, Article ID 5042428, 11 pages Background. D-tagatose is an isomer of fructose and is ~90% as sweet as sucrose with less caloric value. Nowadays
doi: 10.1016/j.jbspin.2017.05.019
Abstract：Although many diagnostic criteria of
Behcet's disease (BD) have been developed and revised by experts, diagnosing BD
is still complicated and challenging. No metabolomic studies on serum have been
attempted to improve the diagnosis and to identify potential biomarkers of BD.
The purposes of this study were to investigate distinctive metabolic changes in
serum samples of BD patients and to identify metabolic candidate biomarkers for
reliable diagnosis of BD using the metabolomics platform. Metabolomic profiling
of 90 serum samples from 45 BD patients and 45 healthy controls (HCs) was
performed via gas chromatography with time-of-flight mass spectrometry
(GC/TOF-MS) with multivariate statistical analyses. A total of 104 metabolites
were identified from samples. The serum metabolite profiles obtained from
GC/TOF-MS analysis can distinguish BD patients from HC group in discovery set.
The variation values of the partial least squared-discrimination analysis
(PLS-DA) model are R2X of 0.246, R2Y of 0.913, and Q2 of 0.852, respectively,
indicating strong explanation and prediction capabilities of the model. A panel
of five metabolic biomarkers, namely, decanoic acid, fructose, tagatose,
linoleic acid, and oleic acid were selected and adequately validated as putative
biomarkers of BD (sensitivity 100%, specificity 97.1%, area under the curve
0.998) in the discovery set and independent set. The principal component
analysis showed clear discrimination of BD and HC groups by the five metabolic
biomarkers in independent set. This is the first report on characteristic
metabolic profiles and potential metabolite biomarkers in serum for reliable
diagnosis of BD using GC/TOF-MS.
Key words：Behcet's disease; Biomarker; Gas
chromatography-mass spectrometry; Metabolomics; Serum